Postdoctoral Fellow

Job Description :

The Tyagi Lab at the University of Louisville is seeking a postdoctoral candidate to investigate cellular and molecular mechanisms underlying the pathophysiology of neurological diseases, such as ischemia stroke and Alzheimer’s disease, and identify novel therapeutic targets for these devastating diseases with a focus on the role of epigenetic regulation, microRNAs, gut-microbiota and exosome on protein regulation and degradation. The candidate should be highly self-motivated and be able to lead a research project. The candidate is expected to participate in writing manuscripts and scientific reports. The candidate should be able to design, execute and troubleshoot experiments and to analyze and interpret results independently. Candidate should have hands-on experience on surgical focal/global ischemia model/or MCAO model is desired. Excellent written and oral communication and organizational skills are essential.

Research Focus :

The successful candidate will utilize advanced unbiased omics approaches—including RNA-Seq, PRO-Seq, ChIP-Seq, and transcriptome—to identify and characterize genetic and epigenetic alterations that contribute to the relationship between proinflammatory signaling and ischemic brain injury. Functional validation will involve both in vitro and in vivo models (middle cerebral artery occlusion), including brain endothelial/microglial cell lines and patient-derived organoids (PDOs).

Essential Duties and Responsibilities :

• Candidate should have hands-on experience on surgical focal/global ischemia model/or MCAO, TBI model is desired.
• Design and conduct experiments using high-throughput sequencing and single-cell technologies.
• Perform molecular biology techniques (e.g., qPCR, Western blotting, immunostaining).
• Analyze transcriptomic, epigenomic, and proteomic data using bioinformatics tools.
• Conduct experiments in rodent models(mouse) and cell cultures.
• Collaborate with team members and contribute to manuscript preparation and grant writing.
• Present research findings at internal meetings and national/international conferences.

Minimum Qualifications :

• PhD or MD/PhD in Neuroscience or a related field.
• Strong background in neurobiology, vascular biology, or epigenetic regulation.
• Experience with middle cerebral artery occlusion (MCAO) and TBI surgery
• Behavior study (memory function) in mouse model is preferred.
• A track record of first-author publications in peer-reviewed journals.
• Excellent communication, organizational, and teamwork skills.

Competencies :

• Ability to work with others and collaborate on projects.
• Excellent writing skills, specifically help PI in grants and research paper publications.
• Candidate should have hands-on experience on surgical focal/global ischemia model/or MCAO model is desired.

Physical Requirements :

• Lab-based work

The above statements are intended to describe the general nature and level of work being performed by individuals assigned to this job. It is not intended to be an exhaustive list of all duties and responsibilities required of individuals in this job. Other duties may be assigned as determined by management. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential duties and responsibilities of this job.

Equal Employment Opportunity: The University of Louisville is an equal opportunity, affirmative action employer, and is committed to providing employment opportunities to all qualified applicants without regard to race, sex, age, color, national origin, ethnicity, creed, religion, disability, genetic information, sexual orientation, gender, gender identity and expression, marital status, pregnancy, or veteran status.

If you require assistance or accommodation with our application process due to an impairment or disability, please email the contact listed above directly, or reach out to the Employment team at or 502.852.6258. Assistance and Accommodations Computers are available for application submission at the Human Resources Department located at 215 Central Avenue, Ste 205 - Louisville, Kentucky 40208.

About the University of Louisville In 2023, the University of Louisville celebrates its quasquicentennial, the 225th anniversary of the 1798 beginnings of higher education in Louisville. One of the nation’s first city-owned, public universities, UofL today is a vital ecosystem that creates thriving futures for students, our community and society. As one of only 79 universities in the United States to earn recognition by the Carnegie Foundation as both a Research 1 and a Community Engaged university, we impact lives in areas of student success and research and innovation, while our dynamic connection with our local and global communities provides unparalleled opportunities for students and citizens both. The university serves as an engine that powers Metro Louisville and the commonwealth and as a classroom for UofL’s more than 23,000 students, who benefit from partnerships with top employers and a wide range of community service opportunities. To learn more, visit louisville.edu. The University of Louisville is a public university in Louisville, Kentucky. It is a member of the Kentucky state university system.